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primary antibodies against collagen 3 alpha 1  (Proteintech)


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    Proteintech primary antibodies against collagen 3 alpha 1
    Primary Antibodies Against Collagen 3 Alpha 1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 710 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+antibodies+against+collagen+3+alpha+1/ENO1+Antibody/pmc10967286-50-4-24
    Average 96 stars, based on 710 article reviews
    primary antibodies against collagen 3 alpha 1 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Immunohistochemistry:

    Article Title: Nucleoredoxin Redox Interactions Are Sensitized by Aging and Potentiated by Chronic Alcohol Consumption in the Mouse Liver
    Article Snippet: .. Next, for IHC analysis, primary antibodies against collagen 3 alpha 1 (COL3A1; 1:200; NB600-594SS, Novus Biologicals; Centennial, CO, USA), interleukin 6 (IL-6; 1:100; 21865-1-AP; Proteintech; Rosemont, IL, USA), KI67 and collagen 1 alpha 1 (KI67; 1:200 and COL1A1; 1:200; GTX16667 and GTX112731, respectively; GeneTex; Irvine, CA, USA), were incubated overnight at 4 °C in the blocking solution provided in the kit (MM510; BioCare Medical). .. After a standard staining protocol using the HRP/DAB Mouse/Rabbit ImmunoDetector Detection System (BSB 0005, BioSB; Santa Barbara, CA, USA), sections were lightly counterstained with hematoxylin, dehydrated, and mounted.

    Incubation:

    Article Title: Nucleoredoxin Redox Interactions Are Sensitized by Aging and Potentiated by Chronic Alcohol Consumption in the Mouse Liver
    Article Snippet: .. Next, for IHC analysis, primary antibodies against collagen 3 alpha 1 (COL3A1; 1:200; NB600-594SS, Novus Biologicals; Centennial, CO, USA), interleukin 6 (IL-6; 1:100; 21865-1-AP; Proteintech; Rosemont, IL, USA), KI67 and collagen 1 alpha 1 (KI67; 1:200 and COL1A1; 1:200; GTX16667 and GTX112731, respectively; GeneTex; Irvine, CA, USA), were incubated overnight at 4 °C in the blocking solution provided in the kit (MM510; BioCare Medical). .. After a standard staining protocol using the HRP/DAB Mouse/Rabbit ImmunoDetector Detection System (BSB 0005, BioSB; Santa Barbara, CA, USA), sections were lightly counterstained with hematoxylin, dehydrated, and mounted.

    Blocking Assay:

    Article Title: Nucleoredoxin Redox Interactions Are Sensitized by Aging and Potentiated by Chronic Alcohol Consumption in the Mouse Liver
    Article Snippet: .. Next, for IHC analysis, primary antibodies against collagen 3 alpha 1 (COL3A1; 1:200; NB600-594SS, Novus Biologicals; Centennial, CO, USA), interleukin 6 (IL-6; 1:100; 21865-1-AP; Proteintech; Rosemont, IL, USA), KI67 and collagen 1 alpha 1 (KI67; 1:200 and COL1A1; 1:200; GTX16667 and GTX112731, respectively; GeneTex; Irvine, CA, USA), were incubated overnight at 4 °C in the blocking solution provided in the kit (MM510; BioCare Medical). .. After a standard staining protocol using the HRP/DAB Mouse/Rabbit ImmunoDetector Detection System (BSB 0005, BioSB; Santa Barbara, CA, USA), sections were lightly counterstained with hematoxylin, dehydrated, and mounted.



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    Extracellular matrix components determination in the liver of aged mice. ( A ) H&E staining. ( B ) M’sT staining. Magnification is 40 and 100× and the scale bar indicates 50 μm. ( C , D ) <t>IHC</t> analysis and signal quantification of COL1A1 <t>and</t> <t>COL3A1</t> proteins. Magnification is 40 and 100× and the scale bar indicates 50 μm. Bars represent mean ± SE. * Significantly different from 7W, ** from 12M compared to the C group, * from 7W, ** from 12M compared to the ALD group, & from 7W, && from 12M, &&& from 18M compared to the respective control group; p < 0.05. n = 5 animals/group. ALD, alcohol liver disease model; C, control; 7W, 7-week-old mice; 12M, 12-month-old mice; 18M, 18-month-old mice.
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    Extracellular matrix components determination in the liver of aged mice. ( A ) H&E staining. ( B ) M’sT staining. Magnification is 40 and 100× and the scale bar indicates 50 μm. ( C , D ) <t>IHC</t> analysis and signal quantification of COL1A1 <t>and</t> <t>COL3A1</t> proteins. Magnification is 40 and 100× and the scale bar indicates 50 μm. Bars represent mean ± SE. * Significantly different from 7W, ** from 12M compared to the C group, * from 7W, ** from 12M compared to the ALD group, & from 7W, && from 12M, &&& from 18M compared to the respective control group; p < 0.05. n = 5 animals/group. ALD, alcohol liver disease model; C, control; 7W, 7-week-old mice; 12M, 12-month-old mice; 18M, 18-month-old mice.
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    Image Search Results


    Extracellular matrix components determination in the liver of aged mice. ( A ) H&E staining. ( B ) M’sT staining. Magnification is 40 and 100× and the scale bar indicates 50 μm. ( C , D ) IHC analysis and signal quantification of COL1A1 and COL3A1 proteins. Magnification is 40 and 100× and the scale bar indicates 50 μm. Bars represent mean ± SE. * Significantly different from 7W, ** from 12M compared to the C group, * from 7W, ** from 12M compared to the ALD group, & from 7W, && from 12M, &&& from 18M compared to the respective control group; p < 0.05. n = 5 animals/group. ALD, alcohol liver disease model; C, control; 7W, 7-week-old mice; 12M, 12-month-old mice; 18M, 18-month-old mice.

    Journal: Antioxidants

    Article Title: Nucleoredoxin Redox Interactions Are Sensitized by Aging and Potentiated by Chronic Alcohol Consumption in the Mouse Liver

    doi: 10.3390/antiox13030257

    Figure Lengend Snippet: Extracellular matrix components determination in the liver of aged mice. ( A ) H&E staining. ( B ) M’sT staining. Magnification is 40 and 100× and the scale bar indicates 50 μm. ( C , D ) IHC analysis and signal quantification of COL1A1 and COL3A1 proteins. Magnification is 40 and 100× and the scale bar indicates 50 μm. Bars represent mean ± SE. * Significantly different from 7W, ** from 12M compared to the C group, * from 7W, ** from 12M compared to the ALD group, & from 7W, && from 12M, &&& from 18M compared to the respective control group; p < 0.05. n = 5 animals/group. ALD, alcohol liver disease model; C, control; 7W, 7-week-old mice; 12M, 12-month-old mice; 18M, 18-month-old mice.

    Article Snippet: Next, for IHC analysis, primary antibodies against collagen 3 alpha 1 (COL3A1; 1:200; NB600-594SS, Novus Biologicals; Centennial, CO, USA), interleukin 6 (IL-6; 1:100; 21865-1-AP; Proteintech; Rosemont, IL, USA), KI67 and collagen 1 alpha 1 (KI67; 1:200 and COL1A1; 1:200; GTX16667 and GTX112731, respectively; GeneTex; Irvine, CA, USA), were incubated overnight at 4 °C in the blocking solution provided in the kit (MM510; BioCare Medical).

    Techniques: Staining, Control

    Effect of chronic alcohol consumption on cell proliferation in liver tissue of young and aged mice. ( A ) IF analysis and signal quantification of PCNA protein. Magnification is 20× and the scale bar indicates 100 μm. ( B ) IHC analysis and signal quantification of KI67 protein. Magnification is 20× and the scale bar indicates 100 μm. Bars represent the mean ± SE. * Significantly different from 7W, * from 7W, ** from 12M compared to the ALD group, & from 7W, && from 12M, &&& from 18M compared to the respective control group; p < 0.05. n = 5 animals/group. ALD, alcohol liver disease model; C, control; 7W, 7-week-old mice; 12M, 12-month-old mice; 18M, 18-month-old mice.

    Journal: Antioxidants

    Article Title: Nucleoredoxin Redox Interactions Are Sensitized by Aging and Potentiated by Chronic Alcohol Consumption in the Mouse Liver

    doi: 10.3390/antiox13030257

    Figure Lengend Snippet: Effect of chronic alcohol consumption on cell proliferation in liver tissue of young and aged mice. ( A ) IF analysis and signal quantification of PCNA protein. Magnification is 20× and the scale bar indicates 100 μm. ( B ) IHC analysis and signal quantification of KI67 protein. Magnification is 20× and the scale bar indicates 100 μm. Bars represent the mean ± SE. * Significantly different from 7W, * from 7W, ** from 12M compared to the ALD group, & from 7W, && from 12M, &&& from 18M compared to the respective control group; p < 0.05. n = 5 animals/group. ALD, alcohol liver disease model; C, control; 7W, 7-week-old mice; 12M, 12-month-old mice; 18M, 18-month-old mice.

    Article Snippet: Next, for IHC analysis, primary antibodies against collagen 3 alpha 1 (COL3A1; 1:200; NB600-594SS, Novus Biologicals; Centennial, CO, USA), interleukin 6 (IL-6; 1:100; 21865-1-AP; Proteintech; Rosemont, IL, USA), KI67 and collagen 1 alpha 1 (KI67; 1:200 and COL1A1; 1:200; GTX16667 and GTX112731, respectively; GeneTex; Irvine, CA, USA), were incubated overnight at 4 °C in the blocking solution provided in the kit (MM510; BioCare Medical).

    Techniques: Control

    Determination of DNA damage and cellular senescence markers induced by chronic alcohol consumption in the liver of young and aged mice. ( A ) IF analysis and signal quantification of PAR protein. Magnification is 20× and the scale bar indicates 100 μm. ( B ) Histochemical analysis and signal quantification of SA-β-gal activity. Magnification is 20 and 40×, and the scale bar indicates 50 μm. ( C ) WB analysis and intensity quantification of pH2A.X protein. pH2A.X protein levels were normalized with those of GAPDH protein, which was used as a loading control. ( D ) IHC analysis and signal quantification of IL-6 protein. Magnification is 20× and the scale bar indicates 50 μm. Bars represent the mean ± SE. * Significantly different from 7W, ** from 12M compared to the C group, * from 7W, ** from 12M compared to the ALD group, & from 7W, && from 12M, &&& from 18M compared to the respective control group; p < 0.05. n = 5 animals/group. ALD, alcohol liver disease model; C, control; 7W, 7-week-old mice; 12M, 12-month-old mice; 18M, 18-month-old mice.

    Journal: Antioxidants

    Article Title: Nucleoredoxin Redox Interactions Are Sensitized by Aging and Potentiated by Chronic Alcohol Consumption in the Mouse Liver

    doi: 10.3390/antiox13030257

    Figure Lengend Snippet: Determination of DNA damage and cellular senescence markers induced by chronic alcohol consumption in the liver of young and aged mice. ( A ) IF analysis and signal quantification of PAR protein. Magnification is 20× and the scale bar indicates 100 μm. ( B ) Histochemical analysis and signal quantification of SA-β-gal activity. Magnification is 20 and 40×, and the scale bar indicates 50 μm. ( C ) WB analysis and intensity quantification of pH2A.X protein. pH2A.X protein levels were normalized with those of GAPDH protein, which was used as a loading control. ( D ) IHC analysis and signal quantification of IL-6 protein. Magnification is 20× and the scale bar indicates 50 μm. Bars represent the mean ± SE. * Significantly different from 7W, ** from 12M compared to the C group, * from 7W, ** from 12M compared to the ALD group, & from 7W, && from 12M, &&& from 18M compared to the respective control group; p < 0.05. n = 5 animals/group. ALD, alcohol liver disease model; C, control; 7W, 7-week-old mice; 12M, 12-month-old mice; 18M, 18-month-old mice.

    Article Snippet: Next, for IHC analysis, primary antibodies against collagen 3 alpha 1 (COL3A1; 1:200; NB600-594SS, Novus Biologicals; Centennial, CO, USA), interleukin 6 (IL-6; 1:100; 21865-1-AP; Proteintech; Rosemont, IL, USA), KI67 and collagen 1 alpha 1 (KI67; 1:200 and COL1A1; 1:200; GTX16667 and GTX112731, respectively; GeneTex; Irvine, CA, USA), were incubated overnight at 4 °C in the blocking solution provided in the kit (MM510; BioCare Medical).

    Techniques: Activity Assay, Control